Polymorphism of growth hormone gene in the artificial insemination result of Madura cattle with Limousin semen  as a reference for genetic selection

E. Safitri Mr,B. Utomo Mr

doi:10.33899/ijvs.2018.153832

Abstract

Research on genetic polymorphism of growth hormone (GH) and receptor growth hormone (rGH) has not been done in crossbred of Limousin cattle, so it is interesting to be examined. Blood samples were taken from 14 Madura calves were artificially inseminated with Limousin cement. DNA amplification is done by using Polymerase Chain Reaction (PCR) method, Restriction Fragment Length Polymorphism (RFLP) method to determine the genotype. DNA sequencing was done to determine nucleotide sequences of GH unit genes. The results showed that identification of GH and rGH gene polymorphisms was done by breaking DNA fragments from 432 and 298 bp in Madura and Limousin cattle (Madrasin) ie, L and V alleles have a frequency of 0.67 and 0.33 for the GH gene, respectively. This proves that the crossed-breeding of Madrasin have V allele that is not owned by the Madura cattle. While in the rGH gene, the A allele is 0.92 and the G allele is 0.08, with the frequency of the A allele larger than the G allele. This research concluded: that GH and rGH undergo changes on polymorphisms in Madrasin cattle can be used as a basis for selection. Keywords:Polymorphism GH Gene rGH Gene Madrasin PCR RFLP V alleles

Highlights

Madura cattle is one of Indonesia's germplasm wealth
Research on genetic polymorphism of growth hormone (GH) gene has not been done in crossbred calves from Limousin cattle, so it is interesting to be investigated deeper so that the results obtained can be used as a reference for obtaining cattle breeds that have a good quality of performance and power reproduction
Research on genetic polymorphism of growth hormone (GH) and β-sub-unit genes has not been done in crossbred calves from Limousin cattle, so it is interesting to obtained can be used as a reference for obtaining a cattle breeds that have a good performance and power reproduction

Summary

Introduction

Madura cattle is one of Indonesia's germplasm wealth. Several laws enacted in an effort to maintain its purity and set out the main points of animal husbandry and animal health, in an effort to maintain the population, shape, colour of the skin, and improve the quality of Madura cattle production.Madura cattle is one type of local Indonesian cattle that grow on the island of Madura and surrounding islands. Genetic studies of Madura cattle that have been crossed with Limousin breed become interesting to do in order to see the calves produced have a good quality in terms of body growth and reproduction.

Results

Conclusion