Gene Polymorphism of Growth Hormone (GH) in One of Indonesian Local Cattle (Madura) and Madura Crossed Cattle

Abstract

The present study was conducted to identify polymorphisms of the growth hormone (GH) gene in one of the Indonesian local cattle breeds (Madura) and Madura crossed (Limousin-Madura) cattle, and also its association with growth traits. Blood samples of 58 cattle were taken from the population of Madura and Madura crossed, then all cattle were located in the same area in Madura island. A 211 bp fragment of the GH gene (spanning from the fourth intron region to fifth exon) was amplified using GHF (forward) primer: 5’GCTGCTCCTGAGGGCCCTTC-3’, and GHR (reverse): 5’CATGACCCTCAGGTACGTCTCCG-3’. Polymorphism was identified with AluI restriction enzyme. The result indicated that two genotypes LL and LV were found at the GH gene in the population of Madura crossed cattle. The frequencies of L and V alleles were 0.64 and 0.04, respectively. Madura and Madura crossed cattle had a significantly differences in their body weight estimation, chest girth, withers height, and body length. The appearance of the LV genotype in Limousin-Madura had no associated effect with body weight estimation and several body measurement yet detected. Keywords: Polymorphism, growth hormone gene, Madura and Limousin Madura cattle.