Abstract
ObjectiveO-linked N-acetylglucosamine (O-GlcNAc) transferase (OGT) catalyzes the addition of O-GlcNAc and GlcNAcylation has extensive crosstalk with phosphorylation to regulate signaling and transcription. Pig OGT is located near the region of chromosome X that affects follicle stimulating hormone level and testes size. The objective of this study was to find the variations of OGT between European and Chinese pigs.MethodsPigs were tested initially for polymorphism in OGT among European and Chinese pigs by polymerase chain reaction and sequencing at the U.S. Meat Animal Research Center (USMARC). The polymorphism was also determined in an independent population of pigs including European and Chinese Meishan (ME) breeds at the National Institute of Animal Science (NIAS, RDA, Korea).ResultsThe intron 20 of OGT from European and Chinese pigs was 514 and 233 bp, respectively, in the pigs tested initially. They included 1 White composite (WC) boar and 7 sows (2 Minzu×WC, 2 Duroc [DU]×WC, 2 ME×WC, 1 Fengzing×WC) at USMARC. The 281-bp difference was due to an inserted 276-bp element and GACTT in European pigs. When additional WC and ME boars, the grandparents that were used to generate the 1/2ME×1/2WC parents, and the 84 boars of 16 litters from mating of 1/2ME×1/2WC parents were analyzed, the breeds of origin of X chromosome quantitative trait locus (QTL) were confirmed. The polymorphism was determined in an independent population of pigs including DU, Landrace, Yorkshire, and ME breeds at NIAS. OGT was placed at position 67 cM on the chromosome X of the USMARC swine linkage map.ConclusionThere was complete concordance with the insertion in European pigs at USMARC and NIAS. This polymorphism could be a useful marker to identify the breed of origin of X chromosome QTL in pigs produced by crossbreeding Chinese and European pigs.
Highlights
O-GlcNAc transferase (OGT) catalyzes the addition of O-GlcNAc and β-N-acetylglucosaminidase (O-GlcNAcase, OGA) is responsible for O-GlcNAc removal, and only these two enzymes are known to regulate GlcNAcylation in mammals [1]
The sequence of OGT intron 20 from DU and White composite (WC) pigs is identical to the OGT sequence that is in the Genome www.ajas.info 1087
For the longer 514-bp intron 20 sequence, numerous homologous regions existed in the pig X chromosome and several other chromosomes including 2, 4, 6, 7, 9, 14, and 16
Summary
O-GlcNAc transferase (OGT) catalyzes the addition of O-GlcNAc and β-N-acetylglucosaminidase (O-GlcNAcase, OGA) is responsible for O-GlcNAc removal, and only these two enzymes are known to regulate GlcNAcylation in mammals [1]. GlcNAcylation has extensive crosstalk with phosphorylation to regulate signaling, transcription and the cytoskeleton in response to nutrients and stress [1]. Nutrient-sensitive enzymes, OGT and AMP-activated protein kinase, cross-talk to mediate cellular metabolism [2]. OGT gene is highly conserved and is localized near the centromeres as a single X-linked gene in mammals [1]. The gene encoding OGT is evolutionarily conserved from plants to humans. In Arabidopsis, OGT is encoded by, SPINDLY (SPY) and SECRET AGENT (SEC) genes, and both genes are required during gameto-
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