Abstract
BackgroundThe present study evaluated levels of polymerization of deoxygenated sickle hemoglobin molecules (poly-dHbS-M) in the presence of fractionated leaf extracts of Anacardium occidentale Linn., Psidium guajava Linn., and Terminalia catappa Linn in vitro as well as identified, quantified, and characterized the phytocomponents from fractionated leaf extracts that exhibited comparatively high potency to impede poly-dHbS-M. Non-hemolyzed sickle erythrocytes were premixed with 40, 60, and 80 mg/100 mL of each of the separate fractionated leaf extracts of A. occidentale, P. guajava, and T. catappa in phosphate-buffered saline (PBS; pH = 7.4), osmotically equivalent to 9.0 g/L NaCl. Poly-dHbS-M was induced by adding 2.0 g/100 mL Na2S2O5 to the erythrocyte suspension. The absorbance of the erythrocyte suspension was measured at regular intervals of 30 s for 180 s. Identification, quantification, and characterization of phytocomponents from fractionated leaf extracts were carried out using GC-MS, FT-IR, and UV-visible systems protocols.ResultsThe level of poly-dHbS-M of the control sample was significantly higher (p < 0.05) than those of the samples containing 40, 60, and 80 mg/100 mL ethylacetate extracts of A. occidentale at t < 60 s. The relative cumulative polymerization index (RCPI%) of dHbS-M in the presence of fractionated leaf extract of A. occidentale varied within a wide range of 3.8–59.4%. A. occidentale (petroleum ether and ethylacetate extracts), P. guajava (n-hexane, chloroform, and ethylacetate extracts), and T. catappa (ethylacetate extract) exhibited comparatively high potency to inhibit poly-dHbS-M.ConclusionThe fractionated leaf extracts of A. occidentale, P. guajava, and T. catappa exhibited differential capacities to impede poly-dHbS-M. The combinations of aliphatic hydrocarbons, methylated esters, methylated fatty acids, aliphatic alcohols, d-erythro-sphinganine, aromatic derivatives, cycloalkanes, phthalates, isothiocyanates, aminated sugars, cyclo-alcohols, and nitro-compounds impeded poly-dHbS-M.
Highlights
IntroductionThe present study evaluated levels of polymerization of deoxygenated sickle hemoglobin molecules (poly-Deoxygenated sickle hemoglobin molecules (dHbS-M)) in the presence of fractionated leaf extracts of Anacardium occidentale Linn., Psidium guajava Linn., and Terminalia catappa Linn in vitro as well as identified, quantified, and characterized the phytocomponents from fractionated leaf extracts that exhibited comparatively high potency to impede poly-dHbS-M
The present study evaluated levels of polymerization of deoxygenated sickle hemoglobin molecules in the presence of fractionated leaf extracts of Anacardium occidentale Linn., Psidium guajava Linn., and Terminalia catappa Linn in vitro as well as identified, quantified, and characterized the phytocomponents from fractionated leaf extracts that exhibited comparatively high potency to impede poly-Deoxygenated sickle hemoglobin molecules (dHbS-M)
Levels of poly-dHbS-M in the presence of fractionated leaf extracts of A. occidentale Figure 1a–e showed the levels of poly-dHbS-M of the control sample and in the presence of fractionated leaf extract of A. occidentale with the progression of experimental time
Summary
The present study evaluated levels of polymerization of deoxygenated sickle hemoglobin molecules (poly-dHbS-M) in the presence of fractionated leaf extracts of Anacardium occidentale Linn., Psidium guajava Linn., and Terminalia catappa Linn in vitro as well as identified, quantified, and characterized the phytocomponents from fractionated leaf extracts that exhibited comparatively high potency to impede poly-dHbS-M. Non-hemolyzed sickle erythrocytes were premixed with 40, 60, and 80 mg/100 mL of each of the separate fractionated leaf extracts of A. occidentale, P. guajava, and T. catappa in phosphate-buffered saline (PBS; pH = 7.4), osmotically equivalent to 9.0 g/ L NaCl. Poly-dHbS-M was induced by adding 2.0 g/100 mL Na2S2O5 to the erythrocyte suspension. Hydrophobic β6valine (Valbeta6) generates a ‘sticky patch’ on the β-globin chains of deoxygenated sickle hemoglobin molecules (dHbS-M) (Martins 1983; Rotter et al 2005). Sodium metabisulfite (Na2S2O5) is often used to induce poly-dHbS-M in vitro, which by virtue of its reducing property triggers low oxygen tension required for aggregation of dHbS-M, engendering morphologically distorted erythrocytes (Oyewole et al 2008; Uwakwe and Nwaoguikpe, 2008; Nurain et al 2017)
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
