Abstract
Two first-generation glutamate (Glu) biosensors are described: one based on a Pt cylinder (125-μm diameter, 1-mm length); the other on a Pt disk (125-μm diameter) with a 30 times smaller surface area. Both designs incorporated the enzyme Glu-oxidase in a polymer (poly-o-phenylenediamine) matrix deposited on the Pt surface. Surprisingly, the smaller disk biosensor displayed a combination of higher Glu current density and lower oxygen dependence compared with the cylinder design. An analysis to estimate the oxygen interference in the Glu signal showed that 90% of the disk biosensor current for 10 μM Glu remains on changing the dissolved oxygen concentration from 200 to 5 μM. These results indicate that brain Glu monitoring in vivo using this design, combined with an enzyme-inactive sensor for differential elimination of electroactive interference, can now be explored without significant influence by fluctuating tissue pO2.
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