Abstract
A novel continuous-flow polymerase chain reaction (PCR) chip has been analyzed in our work. It operates by cycling a prepared sample within three temperature zones. Two temperature zones are controlled by two external PID controllers. And the other is controlled by the flow rate of the fluid inside a water channel under the glass chip. The commercial software is utilized to determine the chip materials that are responsible for creating the denaturation, annealing and extension temperature zones within the chip. Therefore we utilize this PCR chip to perform PCR experiment. And DNA templates provided with the yT&A® cloning vector are amplified successfully.
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