Abstract
Plastic materials are often utilized as plastic packaging and have various uses since their longevity and consistency. Plastic products are generally not biodegradable or persist in our surroundings, posing major harm to the ecology. Biopolymers, or bioplastics, are developing as a blessing in the fight against waste buildup. Polyhydroxybutyrate (PHB) is a biopolymer that can be used instead of manufactured polymers. PHB is a fatty storage substance that accumulates inside microbes' cell walls when stressed. Halophilic microbes can be highly useful in manufacturing PHB since they are cheap, and PHB extraction is considerably easy in halo-tolerant species. As a result, our research emphasizes the identification of PHB-generating halotolerant species of microbes using untreated wastewater. Nile blue A and Sudan Black B staining were used to identify PHB favourable strains. The effective microbe generated PHB on a large scale utilizing sewage as the medium.
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