Isolation and Extraction of PHB from Soil Isolate: Determining its Physio- Chemical characteristics with an application study in Biomedical

Abstract

Biopolymer Poly Hydroxy Butyrate (PHB) is gaining importance based on its physiochemical and biological characteristics. Hence, the characteristics of the PHB extracted from soil bacterial isolate as studied with an aim of developing a 2D films for biomedical applications. The isolates from soil samples were screened for the ability to produce PHB and confirmed using Sudan black staining methods. The physio-chemical characteristics were studied using FTIR, FESEM and GCMS analysis. FTIR spectrum obtained for the extracted polymer shows peaks at 1723.51 cm-1and 1287.63 cm-1 which confirms that the extracted polymer is PHB. FESEM image reveals the presence of pores of different size throughout the sample. The surface consists of multiple pores with different sizes. GC-MS analysis revealed the presence of various compounds attributing for the PHB production from the isolate. As a biological characteristic, a novel wound dressing 2D laminate bio composite films were developed using PHB extracts. The antibacterial activity of the developed film showed significant inhibitory zones against Escherichia coli and Klebsiella pneumoniae. The present research work showed promising results and proved that the PHB obtained from bacterial sp. can be used as an alternative to non-degradable plastics with a wide range of applications in biomedical industries.