Abstract
Poly(ethylene glycol) (PEG) – either PEG 200, 600 or 4000 – when added to the buffer and feed solution gave nearly one-fold increase in the purification and about 100% recovery of recombinant human tumor necrosis factor-α during anion-exchange chromatography. The optimum concentration was 1% with the best result being achieved with PEG 200. © Rapid Science Ltd. 1998
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