Abstract
The polysaccharides of Tremella fuciformis were extracted using an aqueous solution of polyethylene glycol and then separated by ultrafiltration. To obtain the optimal extraction conditions, the effects of different experimental factors on the extraction yield of polysaccharides of T. fuciformis were studied. The experimental results revealed that the optimal polyethylene glycol-based ultrasound-assisted extraction gave a higher extraction yield than that of the common hot water extraction. Using ultrafiltration, the polysaccharides and polyethylene glycol were separated into retentate and permeate solutions, respectively. The recovery and purity of polysaccharides were higher than those of ethanol precipitation. Furthermore, most of polyethylene glycol could be recycled. Therefore, the proposed sample preparation methods were effective in the extraction and separation of polysaccharides, and the recovery of polyethylene glycol.
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