Polyamines do not inhibit erythrocyte ATPase activities

Abstract

To test whether physiologic elevation of red cell polyamine levels might explain Na pump inhibition in sickle cells or uremic red cells, we have studied the effect of putrescine, spermidine and spermine on red cell membrane ATPase and Na-K active transport. Measurement of the ouabain-sensitive influx of 86Rb into intact cells showed no effect of spermine. However, cells became depleted of ATP during incubation with spermine. By 48 h, the cells showed substantial potassium loss and moderate sodium gain. Because the low permeability of red cell membranes for polyamines might have obscured some direct effects on intracellular processes, we measured active transport of 22Na out of red cell ghosts that had been resealed in the presence of 5 mmol/1 spermine. In addition, we measured the Na-K, Mg, and Ca ATPase activities of broken membrane preparations in the presence of spermine, spermidine and putrescine. Polyamines had no direct effect on cation transport in red cells, although possible adverse effects on red cell metabolism could have a secondary effect on cation regulation.