Abstract
Summary The content of several compounds derived from intermediates of arginine metabolism were determined in normal and sulfur-starved cell suspension cultures of Nicotiana tabacum . Of the polyamines putrescine, spremidine, spermine and cadaverine, only putrescine occurs in considerable amounts. Part of the putrescine is derivatized with cinnamic acids. For the determination of caffeoyl- and feruloyl-putrescine a new HPLC method was evolved. It was found that none of these compounds reaches significant level compaired with the great increase of arginine induced by sulfur starvation in photoheterotrophic cells. Further studies of the effect of sulfur starvation on N-accumulation therefore can be focussed on the regulation of the biosynthesis of arginine.
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