Polyamine mediated anti-oxidation activity facilitates of Marsilea minuta L. under Cd toxicity

Abstract

The Marsilea minuta (L) plant was evaluated on the basis of their anti-oxidation path in vitro under Cd mediated metal stress. The plants were grown under varying concentrations of Cd stress and their metal acquisition with concomitant oxidative stress. The different anti-oxidation assay systems were analyzed with regards to putrescine as polyamine interaction and their possible modulation. Thus, Cd treated plants were recorded ABTS and DPPH free radical scavenging activities in terms of 1.75 and 1.59 fold under maximum concentration (200 µM) respectively as compared to control. The spermidine could moderate the activity by 7.53% and 24.91% respectively against the same Cd concentration. On the contrary, a down regulatory trend with a fall in 44.01% activity suggested the chelation efficiency of metal against highest Cd concentration. The other free radical quenching attributes like HOCl - and OH - showed the defense of plants under oxidative stress with records of 1.94 and 2.63 fold over control. In both the cases, spermidine was the moderator by curtailing the activities with 10.70% and 3.18%. Finally, H 2 O 2 , the peroxide crossed the limit of tolerance in tissues with an wider variation by 1.45 fold activity and that was again moderated by spermidine by 10.54%. The different interfaces of anti-oxidations in aquatic fern species was discussed in details taking Cd as pro-oxidant and its possible dealing with polyamine.