POLIMORFIZM RAPD ORAZ ISSR U WYBRANYCH GENOTYPÓW LYCIUM SP.

Abstract

Due to the high value and economic importance of the plant Lycium (goji), its genome has been intensively studied in multidisciplinary research. In the present study, the structure and genetic relationships of 14 selected Lycium genotypes from different origins are presented. By using 18 random amplified polymorphic DNA (RAPD) decamers and 15 inter-simple sequence repeat (ISSR) primers, 200 and 183 loci were amplified, respectively. Among the amplified loci, 45.5–49.2% were polymorphic, and 6.5–7.6% were genotype-specific. Cluster and STRUCTURE analyses performed for RAPD and ISSR revealed the genetic relationships among the genotypes. The highly significant and positive value of the Mantel’s correlation coefficient calculated for the Jaccard similarity matrices of RAPD and ISSR confirmed the suitability of using both these methods separately in this type of study. The significant values of FST statistics obtained in AMOVA for ‘among’ and ‘within’ group analysis confirmed the diversity of genotypes not only between the designated groups but also within them. This diversity provides opportunities to select interesting genotypes and conduct further studies on identifying markers for marker-assisted selection.