Abstract
This study explores the possibility of using polarized Raman spectroscopy for discrimination of cancer from normal tissue. In this paper, polarized Raman spectroscopy (PRS) and polarized Raman imaging (PRI) were used to characterize the isotropic and anisotropic vibrational responses in noncancerous and cancerous human breast tissues. This study has been designed to compare whether more accurate information about cancer and margin measurement can be obtained using PRS and PRI imaging compared to conventional Raman imaging which is the type of imaging currently used. We have shown that the approach presented here opens new possibilities in monitoring biochemical composition, structures and symmetry of vibrations in biological tissues.Additional information gained by using PRS and PRI compared to conventional Raman spectroscopy allow more accurate lesion and margin measurements because the polarized spectroscopy can provide information about molecular orientation and symmetry of the bond vibrations resulting in better visualization of the cancer and normal tissue structures.This study reveals that PRS has better diagnostic potential than the conventional Raman spectroscopic technique. The Raman images of depolarization coefficients give an insight of the modifications of symmetry, orientation, structure and disorder that accompany the transformation of normal cells into cancer cells. In contrast, the isotropic Raman images remove orientational and conformational disorder and provides information on purely vibrational effects, mainly vibrational dephasing. The Raman isotropic lines becomes narrower and the contrast in the isotropic Raman images increases.
Published Version
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