POLARITY GUIDED EXTRACTION AND CHROMATOGRAPHIC IDENTIFICATION OF ACETOGENINS FROM Annona glabra L. LEAVES

Abstract

Annona glabra L. (family: Annonaceae) is used as a traditional medicine 
 against several human infirmities, including cancer. Most of the members of 
 family annonaceae possess a unique class of natural product called 
 acetogenins. The present study was aimed to extract and fractionate the 
 acetogenins from the leaves of Annona glabra using the solvents of increasing 
 polarity, such as hexane, chloroform, ethyl acetate ethanol, and water. Solvents 
 with higher polarity such as ethyl acetate were found more effective in 
 extracting lactone-containing compounds as compared to the less polar 
 solvents. The quantity of acetogenins isolated from the A. glabra leaves was 
 estimated by lactone concentration test. LC-MS/MS screening showed the 
 presence of acetogenins in the samples with m/z values 596.9, 606.9, 679.8, 
 566.8, 550.85, 672.15, 672.15, 624.9 and 565.35. These findings open up 
 effective methods for separation and quantification of bioactive compounds. 
 Further studies are to be focused on evaluating the efficacy of these compounds 
 in pre-clinical and clinical trials, which may potentially contribute in 
 developing the novel therapeutic formulations.