Abstract
Abstract Podoplanin is a mucin-type transmembrane glycoprotein containing extensive O-glycosylation in extracellular domain and two phosphorylation sites in a short cytoplasmic tail. It is expressed in kidney glomerular epithelial cells, lymphatic endothelium and subcapsular epithelial cells in murine thymus. Recently, podoplanin was found on F4/80 positive peritoneal macrophages, indicating that podoplanin is involved in immune response. However, the physiologic function of podoplanin is still unknown. To study whether podoplanin is involved in adaptive immunity, we measured its expression on lymphocyts. The data showed that podoplanin was not detectable on freshly isolated T and B lymphocytes from peripheral blood, spleen and thymus, its expression is also non-inducible after stimulation with PMA, Concanavalin A or LPS. Surprisingly, we found that the podoplanin expression on CD4+ cells was dramatically increased after 3 days culture with Th17 polarization medium compose of immobilized CD3/CD28, IL-6, IL-4, TGFb, IFN-r and IL-23, which showed more than 60% cells were podoplanin positive. However, its expression is not able to be induced on Th1 and Th2 polarized cells. By detecting IL-17 production, we interestingly found that the IL-17+ cells can be divided into podplaninhigh and podoplaninlow populations. The significance of the differential expression on Th17 cells needs to be further investigated.
Published Version
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