Abstract
Background and aims Mesial temporal lobe epilepsy (MTLE) is a particularly devastating form of human epilepsy with significant incidence of medical intractability. MicroRNAs (miRs) are small, noncoding RNAs that regulate post-transcriptional expression of protein-coding mRNAs, which may have key roles in the pathogenesis of MTLE development. We aimed to detect the dynamic expression pattern of brain specific miR-124, miR-134 and miR-9 in the hippocampi of immature rats and children with MTLE. Methods To study the dynamic expression pattern of brain specific miR-124, miR-134 and miR-9, we performed real-time quantitative PCR on the hippocampi of immature rats at 25 days of age. Expression was monitored in the acute, latent, and chronic stages of disease (2 h and 3 and 8 weeks after induction of lithium-pilocarpine status epilepticus, respectively), and in control hippocampal tissues corresponding to the same timeframes. Similar expression method was applied to hippocampi obtained from children with MTLE and normal controls. Results The expression pattern for the miR-124, miR-134 and miR-9 nearly showed the same dynamics in the three stages of MTLE development to be upregulated in the acute and chronic stages and nearly equal to the control in the latent stage, they upregulated also in the children with MTLE. Conclusions The significant upregualtion for the brain specific miR-124, miR-134 and miR-9 in the seizures related stages and children suggested that both can be a potential targets for anticonvulsant drugs in the epileptic developing brains.
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