PO-197 Effects of aerobic exercise training on F13A-mediated energy metabolism in mice

Abstract

Objective Apelin, an adipokine and also a myokine, is involved in energy metabolism. F13A is an analog of apelin-13. In this study, we aim to investigate the effect of aerobic exercise on F13A-mediated energy metabolism in mice.
 Methods 20 C57BL/6J wild mice were randomly divided into 4 groups(n:5), namely saline control group(SC), saline exercise group(SE), F13A control group(FC), and F13A exercise group(FE). Mice were intraperitoneally injected with F13A(0.2 μmol/kg/day) or saline(15 μl/kg/day). Mice in the exercise group underwent 60 min/day treadmill running at a speed of 15 m/min with a slope of 5 º. After 2 weeks, the maximal oxygen uptake was measured and the running speed was adjusted to 20 m/min. The treadmill running continued 4 weeks. The mice were individually housed in a Comprehensive Lab Animal Monitoring System(Columbus Instruments, Columbus, OH, USA) between the 3rd and 4th week of training with free access to food and water. O2 consumption(VO2), CO2 production(VCO2) and respiratory exchange ratio(RER) during a 24-h period were measured after 24h of acclimatization. Glucose oxidation(in g/min/kg0.75=[(4.545×VCO2)−(3.205×VO2)]/1000), and lipid oxidation(in g/min/kg0.75=[1.672×(VO2−VCO2)]/1000) were calculated.
 Results F13A alone increased glucose oxidation(P<0.01, vs SC group). Exercise plus F13A caused a significant decline in RER(P<0.01 vs FC and P<0.05 vs SE group), glucose oxidation(P<0.001 vs FC and P<0.05 vs SE group), whereas it increased lipid oxidation(P<0.05 in comparison with FC group). Exercise alone has no in fluence on 4 groups.
 Conclusions These findings suggest that 4 weeks aerobic exercise can regulate F13A reduce RER in mice, with a decrease of glucose oxidation and an increase of lipid oxidation in vivo.