Abstract
To investigate the effect of testosterone (T) on relaxation of human corpus cavernosum tissue. Human corpus cavernosum tissue samples were obtained after consent from men undergoing penile prosthesis implantation (n=46) for organ bath experiments. Tissue strips were precontracted with phenylephrine after which increasing doses of T, DHT, and androstenedione were added. Blockade of androgen receptor as well as hydrogen sulfide and nitric oxide biosynthesis was studied. Fifteen minutes after addition of the different compounds, tissue relaxation was measured. Results are expressed as mean+/-SD. Statistical analysis was performed using unpaired t-test. T relaxed precontracted tissues with 70.7+/-16.9 % (p = 0.003) at 300 microM compared to DMSO vehicle only. Addition of 300 microM of androstenedione (p = 0.17) and DHT (p=0.09) resulted in no significant difference in relaxation compared to T. Maximum relaxation response to T were not inhibited by N (G)-nitro-L-arginine methyl ester (p = 0.16), beta-cyanoalanine (p = 0.16), propargylglycine (p=0.34) and glibenclamide (p=0.73). The addition of 350 nanoM flutamide (androgen receptor inhibitor) did not alter the effect of T on tissue relaxation with 80.2 +/- 26.5% of relaxation compared to T alone (p = 0.25).
Published Version
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