Abstract
A multimodal therapeutic approach involving radiotherapy is required when treating head and neck squamous cell carcinoma. However, radiotherapy is restricted due to its high risk for damages to the surrounding healthy tissue of the treated area. Tissue regeneration and wound healing is promoted by the survival and regenerative capacities of tissue-resident or invading stem cells. Mesenchymal stem cells (MSCs) exhibit a promising therapeutic potential in the field of cell-based tissue engineering and regenerative medicine due to their immunomodulatory properties and differentiation capacity. However, the generation of MSCs for therapeutic applications is still a major challenge. We aimed to produce highly homogeneous induced pluripotent stem cell-derived mesenchymal stem cells (iP-MSCs) in an autologous manner from initially isolated human mucosa mesenchymal stem cells (mMSCs) of the upper respiratory tract. Therefore, mMSCs were reprogrammed into induced pluripotent stem cells (iPSCs) by non-integrative chromosomal technologies and differentiated into corresponding iP-MSCs. We demonstrated that mMSCs and iP-MSCs show similar cell characteristics in terms of morphology, clonogenic potential, differentiation, and surface phenotype. Moreover, iP-MSCs demonstrated related immunosuppressive capacity as mMSCs including the secretion of cytokines, and T cell inhibition. Therefore, generating iP-MSCs in an autologous manner may be a novel personalized treatment option in regenerative medicine.
Highlights
Introduction nal affiliationsTreatment of head and neck squamous cell carcinoma is based on a multimodal therapeutic approach involving the resection of the tumor, adjuvant radiotherapy, and chemotherapy [1]
We show that human mucosa Mesenchymal stem cells (MSCs) of the upper respiratory tract were isolated and cultivated, reprogrammed into induced pluripotent stem cells (iPSCs) and differentiated into corresponding highly homogeneous induced pluripotent MSCs (Figure 1)
Tissue-derived mesenchymal stem cells (mMSCs) demonstrated characteristic mesenchymal spindle-shaped morphology and the ability to adhere to plastic (Figure 2 (1))
Summary
Introduction nal affiliationsTreatment of head and neck squamous cell carcinoma is based on a multimodal therapeutic approach involving the resection of the tumor, adjuvant radiotherapy, and chemotherapy [1]. Mesenchymal stem cells (MSCs) exhibit a promising therapeutic potential in the field of cell-based, tissue-engineering, and regenerative medicine due to their immunomodulatory properties and differentiation capacity [4]. They are multipotent, non-hematopoietic, plastic adherent fibroblast-like progenitor cells and characterized by certain cell surface markers CD106) [5,6] They have the capability to differentiate into multiple cell lineages including chondrocytes, osteoblasts, adipocytes, tenocytes, or myocytes [7,8]
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