Abstract
The COBE Spectra was evaluated in 71 plateletpheresis procedures. Using the collection and anticoagulant algorithms of the system (n=57) we collected 4.3 ± 1.2×10<sup>11</sup> platelets with a mean separation efficiency of 70.2+12.1%. The cell contamination was very low (leukocytes 0.5 ± 1.0×10<sup>7</sup>, red cells 1.5 ± 2.2×10<sup>7</sup>). In four different modifications of the standard separation protocols, we tried to reduce the ACD consumption in order to shorten the donation time and to improve donor safety. A constant ACD/blood ratio of 1 : 9 and increase of the blood flow to 50ml/min (n=14) caused significantly lower yields (3.1+0.7, p < 0.01) and visible spontaneous platelet aggregates in the collection line in 50% and in the PC’s in 29% of the runs. In orderto prevent platelet activation the ACD algorithm had to be maintained, but a reduction of the ACD/ blood ratio to about 15% was acceptable.
Published Version
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