Platelet Lysate: The Better Choice for Jaw Periosteal Cell Mineralization.

Yvonne Wanner,Siegmar Reinert,Felix Umrath,Marc Waidmann,Dorothea Alexander

doi:10.1155/2017/8303959

Yvonne Wanner, Siegmar Reinert + Show 3 more

Open Access

https://doi.org/10.1155/2017/8303959

Copy DOI

Abstract

Previously, we demonstrated a high quality of minerals formed by serum-free cultured jaw periosteal cells (JPCs) by Raman spectroscopy but the mineralization extent was not satisfactory. In the present study, we analyzed the proliferation and mineralization potential of human platelet lysate- (hPL-) cultured JPCs in comparison to that of FCS-cultured JPCs. By cell impedance measurements, we detected significantly higher population doubling times of PL-cultured JPCs in comparison to FCS-cultured JPCs. However, this result was not based on lower proliferation activities but on diminished cell sizes which JPCs develop under PL cultivation. The measurements of the metabolic activities clearly showed significantly higher cell proliferation rates under PL culturing. Equivalent levels of the mesenchymal cell markers CD29, CD45, CD73, CD90, and CD105 were detected, but there were significantly increased MSCA-1 levels under PL cultivation. While JPCs only occasionally mineralize under FCS culture conditions, the mineralization potential was significantly stronger under PL cultivation. Moreover, in 4 of 5 analyzed patient cells, the addition of dexamethasone was proved no longer necessary for strong mineralization of PL-cultured JPCs. We conclude that in vitro cultivation of JPCs with platelet lysate is a suitable alternative to FCS culture conditions and a powerful tool for the development of high-quality TE constructs using jaw periosteal cells.

Highlights

In order to make clinical applications of tissue engineering constructs safe, we established serum-free culture conditions and observed an earlier but weaker mineralization potential of serum-free cultured jaw periosteal cells (JPCs) [1]
By Raman spectroscopy, we identified and emphasized the differences in the biochemical composition of crystals formed extracellularly under FCScontaining and fetal calf serum (FCS)-free cultivation of JPCs [2]
The diminished extent of JPC calcification as well as the significantly decreased collagen production might lead to an unsatisfactory bone formation significantly countering the success of future tissue engineering applications using this cell type

Summary

Introduction

In order to make clinical applications of tissue engineering constructs safe, we established serum-free culture conditions and observed an earlier but weaker mineralization potential of serum-free cultured JPCs [1]. From the beginning of the cell culture technique up to the present day, the use of fetal calf serum still represents the gold standard for in vitro cell cultivation. Coating of the culture dishes is required for sufficient cell adhesion, the production of extracellular matrix by serum-free cultured cells is normally diminished, and lower cell densities can be achieved. Serum-free cultured JPCs show a reduced mineralization potential, an observation that can partially be explained by the alteration of extracellular matrix formation

Methods

Results

Conclusion