Abstract
Background and Objectives: This study has determined in vitro quality of 5 days stored platelet concentrates prepared by two different methods. Preparation conditions of platelet may cause platelet activation, which contributes to decreased ability of stored platelet to function. The quality platelets concentrate plays an important role in transfusion therapy. Their quality was assessed using the following parameters: platelets, leukocytes and erythrocytes counts, pH, P-selcetin (CD62P) and Annexin V.P-selectin and Annexin V can be detected on the activated platelet. Annexin V was used as a parameter for quality monitoring of platelet concentrates during storage. The present paper compares quality properties of both platelet preparations in vitro. Methods and Materials: In this experimental study, 30 platelet concentrates prepared with platelet rich plasmaplatelet concentrates and 30 units via buffy coat-derived platelet concentrate methods. The percentages of Annexin V, P-selcetin expression, platelet, leukocytes and erythrocytes counts and pH were evaluated. Results: During storage for up to 5 days, buffy coat-derived platelet concentrates units displayed, no significant pH, difference in comparison with platelet rich plasma- platelet concentrates preparation (p>0.05). The mean leukocytes count buffy coat-derived platelet concentrates and platelet rich plasma- platelet concentrates was comparable and statistically significant difference was observed (p<0.05). During storage for up to 5days platelet rich plasma- platelet concentrates units displayed significant an increase in the CD62P, Annexin V expressions, as compared with buffy coat-derived platelet concentrates preparation on day 5 (p<0.05). Conclusions: The kinetics of CD62P and annexin V levels are influenced by the method used to prepare platelets for storage. The different levels of CD62P and annexin V in buffy coat-derived platelet concentrates and platelet rich plasma- platelet concentrates clearly demonstrating a progressive activation process of platelet rich plasma- platelet concentrates exceeds that of buffy coat-derived platelet concentrates.
Highlights
Preparation conditions and storage of platelet for transfusion may cause platelet activation, which contributes to decreased ability of stored platelet to function and to survive in vivo after transfusion compared with that seen with freshly prepared platelets [1,2,3,4]
The kinetics of CD62P and annexin V levels are influenced by the method used to prepare platelets for storage
Quality of the platelet concentrates was assessed by cell counts/bag, pH, CD62Pand Annexin V
Summary
Preparation conditions and storage of platelet for transfusion may cause platelet activation, which contributes to decreased ability of stored platelet to function and to survive in vivo after transfusion compared with that seen with freshly prepared platelets [1,2,3,4]. We here report on the difference between both platelet concentrates in functional properties of platelet in BC and PRP-PC are compared over storage of 5 days. The aim of this study is to characterize the quality of the changes occurred in the platelet activation parameters during the storage of platelet concentrates derived from BCs and PRPPCs. This study has determined in vitro quality of 5 days stored platelet concentrates prepared by two different methods. The quality platelets concentrate plays an important role in transfusion therapy. Their quality was assessed using the following parameters: platelets, leukocytes and erythrocytes counts, pH, P-selcetin (CD62P) and Annexin V.P-selectin and Annexin V can be detected on the activated platelet. Annexin V was used as a parameter for quality monitoring of platelet concentrates during storage. The present paper compares quality properties of both platelet preparations in vitro
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