Plasticity of the sympathetic nervous system innervating the cerebral arteries in rats

Abstract

In order to investigate the neuroplasticity of the peripheral sympathetic nervous system innervating the cerebral blood vessels, we observed and traced the sprouting nerve fibers originating in the contralateral superior cervical ganglion (SCG) into the previously denervated cerebral arteries following unilateral excision of the SCG and/or decentralization of the contralateral SCG in young rats (4 weeks old). These nerve fibers were labeled anterogradely with wheat germ aggulutinin-horseradish peroxidase or stained immunohistochemically with anti-tyrosine hydroxylase antibody. Eight weeks after the right SCG excision, reinnervating nerve fibers originating in the contralateral ganglion formed a circular pattern of nerve plexus only on the wall of the main cerebral arteries of the circle of Willis in the ganglionectomized side. However, the decentralization of the contralateral SCG, which was performed simultaneously with a unilateral SCG excision, prevented the nerve sprouting into the denervated regions. Unilateral decentralization of SCG itself failed to affect their distribution pattern or their density of nerve fibers originating in the ganglion. It is concluded that in the young rat the outgrowth of the sympathetic nerve fibers into the denervated cerebral arteries was strongly impeded by the disconnection of ganglion cells from the central nervous system, while the decentralization alone could not affect the innervation pattern of the postganglionic fibers which have been already built-up in the cerebral arterial system.