Plasmonic gold nanoparticles aggregate based on charge neutralization for the convenient detection of fumonisin B1 by colorimetry and SERS

Abstract

A novel charge neutralization strategy with positively charged SYBR Green I was used for the convenient detection of fumonisin B1. In the absence of the fumonisin B1, the aggregation of gold nanoparticles would appear in less than 2 min. In the presence of the fumonisin B1, the positive charge of SYBR Green I was neutralized by the negatively charged fumonisin B1. As a result, the aggregation of gold nanoparticles could not be achieved. Based on the charge neutralization strategy, the colorimetry and surface enhanced Raman spectroscopy (SERS) dual-channel methods were developed for the detection of fumonisin B1 in corn. The naked eye sensitivity was 500 μg kg−1 in the colorimetric method. The limit of detection was 54.86 μg kg−1 in the SERS method. The developed methods had a high stability (coefficient of variation <8.3%).