Abstract
Actively motile bacteria became non-motile when plasmolyzed and they regained their motile function when deplasmolyzed. Many of the species observed spontaneously reverted to motile forms if allowed to remain sufficiently long in the plasmolyzing medium. The time required for resumption of motility could be decreased markedly by addition of tryptone, or dl-alanyl-dl-serine, or l-alanine to the medium. Motility resumption occurred when the bacteria stained in a manner similar to that of non-plasmolyzed forms, that is, evenly rather than bipolarly, thus suggesting that the displacement of the membrane influenced motile function.
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