Plasmatic TXB 2 and 6-keto-PGF 1α levels during charcoal hemoperfusion in chronic renal failure patients

Abstract

6 patients with end-stage renal disease underwent hemoperfusion with charcoal colums, for 60 min. Blood samples anticoagulated with 2% EDTA/aspirin solution were obtained from arteriovenous fistulas in the basal condition, 5 min after a bolus injection of heparin (7,500 U), at the end of hemoperfusion, and 30 min after. The study was repeated few days later, in the same patients, two hours after 100 mg aspirin by mouth. TXB 2 and 6-keto-PGF 1α were assayed with RIA in unextracted (U) and extracted (E) and chromatographed platelet poor plasma (PPP). Platelet counts before and after hemoperfusion were also performed. Low levels of the two prostaglandins were found in plasma; this could be related to the procedures for collection and processing of plasma samples; no significant differences were observed between extracted and unextracted samples: there were slightly higher levels of 6-keto-PGF 1α in unextracted samples. After charcoal hemoperfusion there was only a slight and not significant increase of TXB 2 and 6-keto-PGF 1α; low dose aspirin did not modify significantly plasma levels of the two prostaglandins before hemoperfusion but it reduced TXB 2 and 6-keto-PGF 1α levels after charcoal hemoperfusion. The platelet count fell (−22%) after charcoal hemoperfusion with heparin alone and in a similar manner after lowdose aspirin pretreatment (−24,7%).