Abstract
To the Editor: Bystrom et al. described an angiotensin I accumulation assay by LC-MS/MS to measure plasma renin activity (PRA),1 which included an isotope-labeled degradation standard to monitor the stability of angiotensin I (1). In this analysis, a small group of patient samples demonstrated “fast angiotensin I degradation” activity during the 3-h incubation period. Their findings indicated that angiotensin I concentrations could be significantly affected by this degradation and were therefore not an accurate marker for PRA in these samples. Further study of these degradation results indicated that ( a ) they were patient specific, which was confirmed by repeat draw and analysis; ( b ) the degradation activity was not inhibited by the addition of EDTA, indicating that the degradation activity was independent of calcium concentration; ( c ) samples with degradation obtained with and without addition of neomycin were highly correlated, suggesting that bacterial contamination …
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