Abstract
Obesity acts as a cardiovascular risk factor by mechanisms that are not fully understood. Osteopontin (OPN) is a proinflammatory mediator involved in tissue remodeling that plays a role in atherosclerosis and diabetes. The aim of the present study was to compare the circulating concentrations of OPN and its mRNA expression in omental adipose tissue of lean, overweight, and obese individuals and to analyze the effect of weight loss. Plasma concentrations of OPN were measured in 77 volunteers. OPN mRNA expression in omental adipose tissue obtained from 12 women was quantified by real-time PCR. In addition, the concentrations of OPN in 12 obese men were measured before and after weight loss following a dietetic program. The study was conducted at a University Hospital. Obese and overweight patients exhibited significantly increased circulating OPN concentrations as compared with lean subjects (obese 72.6 +/- 28.5, overweight 68.2 +/- 20.8, lean 42.7 +/- 27.9 ng/ml; P < 0.001). A significant positive correlation was found between OPN levels and body fat (r = 0.45; P < 0.0001). Obese individuals showed significantly increased (P < 0.05) mRNA expression of OPN in omental adipose tissue as compared with lean volunteers, which was further increased in obese diabetic patients. Diet-induced weight loss significantly decreased OPN concentrations from 64.7 +/- 22.1 to 36.6 +/- 20.1 ng/ml (P = 0.006). These findings represent the first observation that plasma OPN and mRNA expression of OPN in omental adipose tissue are increased in overweight/obese patients with the latter being further elevated in obesity-associated diabetes. Moreover, weight loss reduces OPN concentrations, which may contribute to the beneficial effects accompanying weight reduction. Measurement of OPN might be useful for evaluating the outcomes of various clinical interventions for obesity-related cardiovascular diseases.
Highlights
Diet-induced weight loss significantly decreased OPN concentrations from 64.7 Ϯ 22.1 to 36.6 Ϯ 20.1 ng/ml (P ϭ 0.006). These findings represent the first observation that plasma OPN and mRNA expression of OPN in omental adipose tissue are increased in overweight/obese patients with the latter being further elevated in obesity-associated diabetes
Weight loss reduces OPN concentrations, which may contribute to the beneficial effects accompanying weight reduction
OBESITY IS ASSOCIATED with alterations in myocardial and vascular structure as well as physiology, which are accompanied by an adverse risk factor profile leading to the development of cardiovascular disease [1,2,3]
Summary
Plasma concentrations of OPN were measured in 77 volunteers. The concentrations of OPN in 12 obese men were measured before and after weight loss following a dietetic program. Setting: The study was conducted at a University Hospital. To analyze the effect of obesity on OPN concentrations, 77 Caucasian subjects (26 lean, 14 overweight, and 37 obese) were recruited from healthy volunteers and patients attending the Endocrinology and Surgery Departments at the University Clinic of Navarra. Subjects were classified according to body mass index (BMI) (lean, Ͻ25; overweight, 25–29.9; and obese, Ն30 kg/m2). Patients underwent a clinical assessment including medical history, physical examination, body composition analysis, and comorbidity evaluation as well as nutritional interviews performed by a multidisciplinary consultation team. Obese patients were not receiving statins or antidiabetic medication. Normoglycemia (NG), impaired glucose tolerance (IGT), and type 2 diabetes mellitus (T2DM) are defined following the criteria of the Expert Committee on the Diagnosis and Classification of Diabetes Mellitus [22] based on both fasting plasma glucose concentrations and plasma glucose 2 h after an oral glucose tolerance test (OGTT)
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