Abstract

Summary Cotyledons, hypocotyls, leaflets and shoot apices of Mimosa pudica, cultured on B5 medium comprising of an auxin, 2,4-D (0.5 ppm), and a cytokinin, BAP (1 ppm), showed high percentage of callusing in all the expiants within a few weeks of culture. After about 2 months of culture, multiple shoot buds differentiated from these which could be successfully rooted on BM or BM + IAA (2 ppm) to form complete plantlets.

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