Abstract
Management of forest lands includes reforestation, ideally with superior propagules. Tissue culture technology, one approach to obtaining superior clones, is now being applied to conifers. Excised embryos and young seedling parts of Pinus rigida were induced to form multiple shoots when cultured on defined media containing a cytokinin. The age of the explants and the composition of the culture medium significantly influenced the frequency of adventitious bud formation. Temperature and photoperiod during culture also strongly influenced the differentiation and development of the buds. The elongation of the buds was achieved by elimination of cytokinin, reducing the concentration of salts and carbohydrate and addition of activated charcoal to the medium. Rooting was induced by treating the shoots with indole-3-butyric acid and naphthalene acetic acid for 5 days before transfer to auxin-free medium. The regenerated plantlets were then transferred to soil under non-sterile conditions for further development.
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