Abstract
Although multiple lines of evidence have indicated that Arabidopsis thaliana Tandem CCCH Zinc Finger proteins, AtTZF4, 5 and 6 are involved in ABA, GA and phytochrome mediated seed germination responses, the interacting proteins involved in these processes are unknown. Using yeast two-hybrid screens, we have identified 35 putative AtTZF5 interacting protein partners. Among them, Mediator of ABA-Regulated Dormancy 1 (MARD1) is highly expressed in seeds and involved in ABA signal transduction, while Responsive to Dehydration 21A (RD21A) is a well-documented stress responsive protein. Co-immunoprecipitation (Co-IP) and bimolecular fluorescence complementation (BiFC) assays were used to confirm that AtTZF5 can interact with MARD1 and RD21A in plant cells, and the interaction is mediated through TZF motif. In addition, AtTZF4 and 6 could also interact with MARD1 and RD21A in Y-2-H and BiFC assay, respectively. The protein-protein interactions apparently take place in processing bodies (PBs) and stress granules (SGs), because AtTZF5, MARD1 and RD21A could interact and co-localize with each other and they all can co-localize with the same PB and SG markers in plant cells.
Highlights
IntroductionProtein machineries govern almost all cellular processes in response to internal and external cues
In living organisms, protein machineries govern almost all cellular processes in response to internal and external cues
Results of Gene Ontology (GO) analysis indicated that 19 out of 35 candidates were involved in different types of stress responses including salt, oxidation, cold, heat, ABA, pathogens and hypoxia (Fig 1B and S3 Table)
Summary
Protein machineries govern almost all cellular processes in response to internal and external cues. It is rare that proteins act alone, instead, they interact with each other to orchestrate biological functions, such as transcriptional control of gene expression [1], cellular signal transduction [2], intracellular protein trafficking [3], cell cycle progression [4,5,6] and various types of cellular metabolism [7]. AtTZF4, 5, and 6 Interact with Stress Responsive Proteins
Sign-in/Register to view highlights & summary 
Published Version (
Free)
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call