Abstract
We have established a high-frequency plant regeneration system via somatic embryogenesis from seed-derived callus and cell suspension cultures in 6 genotypes of bahiagrass ( Paspalum notatum). Embryogenic callus was initiated from mature seeds on Murashige and Skoog (MS) medium supplemented with 2.0 mg/l 2,4-dichlorophenoxyacetic acid (2,4-D), 3.0% sucrose and 0.3% Gellan Gum in the dark. Culture response was found to be correlated with genotype. ‘Pensacola’ had the best response in embryogenic callus formation, and 74% of the calli regenerated plants. Cell suspension cultures were established from embryogenic callus of ‘Pensacola’ in modified N 6 medium containing 1.0 mg/l 2,4-D. The suspension was composed of compact cell clusters. When smaller clusters (approx. 2.0–3.3 mm in diameter) were transferred to a solid MS medium with 3.0% sucrose but without hormones, plant regeneration was initiated at high frequency (28.6%). Morphological evidence is provided that regeneration of suspension cells occurred via embryogenesis. Regenerated plants were established to soil after culture in water at room temperature for 14 days for acclimatization.
Published Version
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