Abstract
A new method has been developed for the obtention of protoplasts of potato ( Solanum tuberosum L.) diploid clones and for the regeneration of whole plants. This procedure determined specific conditions of in vitro culture of shoots: the propagation medium, light intensity and length of illumination period. Four successive media from the protoplast level to the shoot formation have been optimized. This method is suitable for the obtention of calli from 13 diploid potato lines, among 15 tested. Regeneration of plants has been obtained for 10 clones with efficiencies from 0.5 to 4 plants regenerated from 100 plated protoplasts.
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