Abstract
Gibberellin A 12(GA 12), GA 15 and GA 20 were identified by full scan GC-MS from a culture filtrate of Phaeosphaeria sp. L487, in addition to the previously identified GAs, GA 1, GA 4, GA 9, GA 24,and GA 25. The early-non-hydroxylation pathway from GA 12-7-aldehyde to GA 1 was shown to be present in the fungus by feeding studies of [1,2- 3H 2]GA 4, [17- 14C]GA 9, [17- 13C, 3H]GA 12-7-aldehyde and [17- 13C, 3H]GA 20. The fungus metabolized GA 9 to GA 1 via GA 4 and GA 20. Comparison of the conversion of GA 4 and GA 20 to GA 1 based on HPLC analysis indicated that the conversion of GA 4 to GA 1 was more effective than that of GA 20 to GA 1. The pathway from GA 9 to GA 1 via GA 4 and GA 20 is novel in microorganisms and like that of some higher plants.
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