Pioneer trees of Betula pendula at a red gypsum landfill harbour specific structure and composition of root-associated microbial communities

Abstract

The study of root-associated microbial communities is important to understand the natural processes involved in plant recolonisation at degraded areas. Root associated bacterial and fungal communities of woody species colonising a red gypsum landfill (a metal-enriched environment) were characterised through metabarcoding. Among trees naturally growing on the landfill, Betula pendula is the only tree species in the centre of the area, whereas companion tree species such as Populus nigra, P. tremula and Salix purpurea were present on the edges. The bacterial community was dominated by Proteobacteria (38%), Actinobacteria (35%) and Bacteroidetes (20%) and the most abundant bacterial OTU belonged to the family Streptomycetaceae. The fungal community was dominated by Ascomycota (60%) and Basidiomycota (30%) and the most abundant family was Pyronemataceae. Analysis of similarities, heatmap and hierarchical cluster analysis showed that B. pendula grown in the centre of the landfill harboured a specific microbial community, which was unique and different, not only from other tree species (Populus or Salix spp.), but also from other B. pendula growing at the edges. Our findings on relevant indicator OTUs associated to the birches located in the centre of the landfill (such as Otu00716 Catellatospora sp. (family Micromonosporaceae, phylum Actinobacteria) or Otu4_35502 Russula sp. (family Russulaceae, phylum Basidiomycota)) may have important implications for the successful revegetation of these harsh environments using microbial-based phytostabilisation approaches.