P‐II‐05 Effects of BMP‐2 Gene and bFGF Protein Combined with Porous Hydroxyapatite on Bone Formation

Abstract

In this study, the bone defects created on animal cranium which treated by BMP gene (cDNA plasmid) and bFGF protein introduced with porous HAP and the bone formation was analyzed histpathologically. The bone defect of 1.2 cm in the diameter was made on rabbit's cranium and HAP pellets of exactly 1.0 cm in diameter were implanted after completion of hemostasis. The amount of the bone formation was compared with or without the presence of the BMP‐2 cDNA plasmid. In addition, simultaneous administration of bFGF containing solution with or without BMP gene were performed to analyze the effect of combined use of them. The bone formation was vigorously observed in the cranial defect in the groups, which received the BMP‐2 gene with HAP at three weeks after the operation, and complete ossification was observed at 9 weeks after the operation. In the HAP which containing BMP‐2 gene implanted group, although it revealed that new bone formation was evident surrounding the HAP pellets at three weeks after the operation, the inducted bone tissue did not fill to the entire pores of the HAP pellets even at 9 weeks after the operation which condition was improved by coadministration of bFGF with BMP gene. Those results show that at the BMP gene therapies the bone formation in the pores of the HAP pellet was promoted by coadministration of bFGF. It is possible the effects of the administration of the BMP‐2 gene will be improved, from the above‐mentioned result, through coadministration of bFGF and BMP gene.