Abstract
AbstractFor the performance assessment of an electrochemical flow cell using a boron‐doped diamond (BDD) electrode in high‐performance liquid chromatography with electrochemical detection (HPLC‐ECD) to determine cholesterol, the standard deviation (SD) of area measurements in chromatographic baseline noise (AMCBN) was evaluated based on the function of mutual information (FUMI) theory without repetitive chromatographic measurements. After the selection of suitable components for the electrochemical flow cell in HPLC‐ECD, the detection limit of cholesterol was 0.04 pmol at a detection potential of +2.2 V vs. an Ag/AgCl electrode. The present HPLC‐ECD was applied to determine cholesterol in various meats.
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