Picomolar level sensorial dual colorimetric gold nanoparticle sensor for Zn2+ and Hg2+ ions synthesized from bark extract of Lannea Grandis Coromandelica and its wide range applications in real sample analysis

Abstract

In this work a facile, rapid, reproducible and non-toxic approach has been demonstrated for synthesis of most stable AuNPs from bark extract of Lannea Grandis Coromandelica. UV–Visible spectroscopy, FTIR, TEM, SAED, EDX, XRD, DLS, Zeta Potential, FE-SEM, AFM and XPS techniques were employed for the characterization of synthesized LGC-AuNPs. The UV–Vis spectra of LGC-AuNPs gave SPR peak at 536 nm while the TEM analysis revealed LGC-AuNPs have 20.75 nm size with spherical in shape. DLS study showed the AuNPs have average diameter 50.18 nm. The synthesized AuNPs exhibited very high selectivity, rapid response in recognition towards Zn2+ and Hg2+ ions by changing its color within 20 sec. This proposed sensor can detect very low picomolar level of Zn2+ and Hg2+ ions (LOD value for Zn2+ and Hg2+ were found 1.36 pM and 24.60 pM respectively). Here we also studied effect of several factors such as variation of conc of gold, temperature, incubation time, pH, salt, solvent (polar protic and polar aprotic) to know in which condition AuNPs have high stability and sensitivity. The data revealed that synthesized AuNPs was stable up to two years at pH 6.5 at room temperature in water media and under this condition, it shows maximum sensitivity and reactivity. Moreover, here interference study was carried out to identify high selectivity of synthesized LGC-AuNPs probe in presence of different metal ions. The real sample analyses also revealed the great applicability of this probe. Therefore, this simple, rapid, low-cost, sensing activity appeared to hold great sensibleness for detection of heavy metal ions in real sample.