Abstract
Using a new type of DNA and carbon nano tube (CNT) mixed paste electrode using cyclic and square wave anodic stripping voltammetric (SWASV) methods, this study presents an assay of riboflavin (RF) under optimum conditions. Results of the experiment yielded a low working concentration range of nanograms with 1–10 and 10–170ngL−1 and 5–105μgL−1, at an accumulation time of 80s in a 0.1M H3PO4 electrolyte solution. A relative standard deviation of 30μgL−1 was observed at an accuracy level of 0.1164% (n=15) under optimum conditions. The detection limit (S/N) was pegged at 0.2ngL−1 (5.31×10−13molL−1 RF). The proposed method was successfully applied to an actual human urine and drug sample, and can be applied to assays of other biological samples.
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