Phytotoxicity of tris-(1-chloro-2-propyl) phosphate in soil and its uptake and accumulation by pakchoi (Brassica chinensis L. cv. SuZhou)

Abstract

This study investigated physiological and biochemical changes in pakchoi at different growth stages (25 and 50 d) under different tris-(1-chloro-2-propyl) phosphate (TCIPP) treatments (10, 100, 500, and 1000 μg kg−1). The uptake and accumulation of TCIPP by pakchoi and variation of TCIPP speciation in soil were also determined. TCIPP decreased the length and fresh weight of pakchoi root compared with those in blank controls, and this effect was significant when the concentration of TCIPP was higher than 100 μg kg−1. The fresh weight of pakchoi stems and leaves, the chlorophyll content, and the activities of superoxide dismutase, peroxidase, and catalase in the leaves first increased and then decreased with increasing TCIPP concentration. The inflection point of the variation in these indices was 100 μg kg−1 TCIPP in soil. The contents of proline and malondialdehyde increased continuously with increasing TCIPP concentration. The uptake of TCIPP by pakchoi increased linearly with increasing TCIPP concentration, and the highest TCIPP concentrations in the roots, stems, and leaves were 275.9, 80.0, and 2126.3 μg kg−1, respectively. TCIPP was easily transferred from the roots to leaves of pakchoi, with translocation factor of up to 12.6. The content of bioavailable TCIPP in soil was high, accounting for 46.5%. Planting pakchoi could significantly reduce the content of bioavailable TCIPP, with removal rate of 39.9%–54.1%. After 50 d of planting pakchoi, the removal rate of TCIPP in soil (10.4%–18.6%) was significantly higher than that in the control without plant, but the contribution of phytoextraction was small, accounting for 2.62%–26.6%.