Phytotoxic effect of Carica papaya extract on seed-borne fungus of African Yam Bean (Sphenostylis stenocarpa Hochst ex. A. Rich. Harms) seeds

Abstract

Extracts of many higher plants have been reported to exhibit antifungal properties under laboratory experiments. This study was therefore carried out to investigate the effect of Carica papaya plant extracts on the African Yam Bean seed-borne pathogen. The seed health test of African Yam Bean was carried out using the blotter paper method. The experiment was conducted at the Plant Pathology Laboratory of the Department of Crop Science and Horticulture, Nnamdi Azikiwe University, Awka. Test of plant extract for inhibition of radial growth of seed-borne pathogen of African yam bean was studied under in vitro experiment at 0%, 50%, 75% and 100% concentrations with 0% as the control. The design was a Completely Randomized Design (CRD) with three replications. The test plant extracts of different concentrations were added into Petri dishes containing molten-sterilized Sabouraud Dextrose Agar (SDA) and swayed gently on the laboratory bench to allow an even mixture These were allowed to gel. Then nine millimeter discs of a seven-day pure culture of Aspergillus flavus were aseptically placed on the centre of the Petri dishes containing the SDA-extract mixture. Records on the radial inhibition effect of the test plant extract were kept for further analysis. Data on radial inhibition of fungus were subjected to Analysis of Variance (ANOVA). Results showed that Aspergillus niger, Aspergillus flavus and Aspergillus terreus were isolated from incubated African Yam Bean seeds. Carica papaya leaf extract used was very effective and the higher the concentrations of extract, the more effective in the inhibition of radial growth of the test fungus. It could therefore be recommended that farmers should always conduct viability tests on procured seeds before planting. Farmers should rather use plant extract such as the one used in this experiment in controlling seed-borne fungal pathogens of African Yam Bean seeds than synthetic fungicides.