Phytotoxic activity of compounds from Moutabea guianensis aubl. on Amazonian invasive species

Haroldo Da Silva Ripardo Filho,Oscar A S Romero,Lourivaldo S Santos,Marivaldo J C Corrêa,Edinaldo S Andrade,Giselle M S P Guilhon,Luidi C Pacheco,Antonio Pedro Da S Souza Filho,Williams Da S Ribeiro

doi:10.26850/1678-4618eqj.v40.1.2015.p72-76

Haroldo Da Silva Ripardo Filho, Oscar A S Romero + Show 7 more

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https://doi.org/10.26850/1678-4618eqj.v40.1.2015.p72-76

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Abstract

This study aimed establish the variations in the phytotoxic activity of hexane, ethyl acetate and methanol extracts of Moutabea guianensis, and methyl caffeate and scopoletin isolated from the ethyl acetate extract, variyng the concentration and the receptor species. Phytotoxic activity bioassays of germination (at 25 °C and 12 hours of photoperiod) and development of radicle and hypocotyl (25 °C and 24 hours of photoperiod) were developed. The seed germination of Mimosa pudica was sensitive to the roots hexane, ethyl acetate and methanol extracts at 1% (w/v), with inhibition potentials in 92%, 100% and 100%, respectively. Comparative analysis on the phytotoxic activity of the tested compounds revealed that scopoletin showed a higher inhibition potential on the seed germination bioassay against Mimosa pudica. Senna obtusifolia was not sensitive to the tested compounds. Methyl caffeate showed the highest potential to inhibit the development of radicle and hypocotyls, and the intensity of the allelopathic effects varied with the concentrations.

Highlights

An alternative for replacing the current forms of weed control has been using plants and microorganisms for obtaining phytotoxic compounds, to be used directly as herbicides, or as prototypes for discovering new synthetic herbicides [1,2]
Coumarins represent a kind of substances with inhibitory activity reported on the seed germination [16], and the phenylpropanoids and its derivatives have been reported in the literature for presenting phytotoxic activity [17,18]
From the analysis of the 1H and 13C NMR spectra and subsequent comparison with data obtained from the literature (Table 1 and 2), compounds 1 and 2 were identified, respectively, as methyl caffeate [24] and scopoletin [25] (Figure 1)

Summary

Introduction

An alternative for replacing the current forms of weed control has been using plants and microorganisms for obtaining phytotoxic compounds, to be used directly as herbicides, or as prototypes for discovering new synthetic herbicides [1,2]. The natural phytotoxins present wide structural diversity with compounds belonging to various classes of substances [4]. Moutabea guianensis is an Amazonian species that belongs to the family Polygalaceae, commonly known as cipó gogó de guariba [5]. From the roots of M. guianensis were isolated two compounds, the phenylpropanoid derivative methyl caffeate (1) and the coumarin scopoletin (2). Coumarins represent a kind of substances with inhibitory activity reported on the seed germination [16], and the phenylpropanoids and its derivatives have been reported in the literature for presenting phytotoxic activity [17,18]. As part of a program on Amazonian plants with allelopathic and phytotoxic activity [19,20], in this work we report the phytotoxic activity of extracts and compounds from the roots of Moutabea guianensis against two invasive plants Mimosa pudica (malicia) and Senna obtusifolia (mata-pasto)

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