Abstract
To prevent microbial growth, chemical solvents are typically utilized. However, chemical solvents are hazardous to human health with low antimicrobial effects. Metallic-element (such as copper, silver, and gold) nanoparticles have many applications in biotechnology and biomedicine. Copper nanoparticles (CuNPs) are efficient owing to their antimicrobial, anti-inflammatory, and anti-proliferative properties. The objective of this study was to perform biogenic synthesis of copper nanoparticles using three different spices (star anise, seed of Illicium verum; nutmeg, seed of Myristica fragrans; and mace, membrane covering the seed of Myristica fragrans) and determine their antibacterial properties. CuNPs of spices were prepared by dissolving copper sulfate in the respective plant extract. They were then characterized by UV-Vis spectroscopy, FTIR, GC-MS, EDAX, and SEM analysis. Results of UV-Vis spectroscopy showed the maximum absorbance peak at 350 nm. SEM analysis showed that the sizes of these CuNPs were in the range of 150–200 nm. EDAX analysis confirmed the presence of copper and oxygen and revealed that copper existed in an oxidized form. FTIR spectroscopy showed the presence of different functional groups in these synthesized nanoparticles. GC-MS analysis revealed compounds such as Anethole D-limonene, heptadecanoic acid, 16-methyl-, methyl ester, myristene, methyl eugenol, and methyl stearate, indicating the presence of functional groups. The antibacterial activities of the three extracts from spices were analyzed using growth zone inhibition and TLC-bioautography methods. The results showed that star anise spice extract had the highest antibacterial activity. These results indicate that such CuNPs phyto-formulated with spice extracts having antibacterial properties could be used as potential therapeutics for microbial diseases.
Highlights
In modern years, there has been a continuing renewal of interest pertaining to the utilization of therapeutic and aromatic plants to produce antimicrobial drugs as plantderived drugs are assured to be harmless and without side-effects [1]
CuNPs synthesized with Syzygium aromaticum bud extract against different including Bacillus subtilis (ZOI = 4.2 cm) and Escherichia coli (ZOI = 3.3 cm) at a concentration bacterial species including Bacillus subtilis (ZOI = 4.2 cm) and Escherichia coli (ZOI = 3.3 cm) of 200 μg/mL [26]
We instituted a phytoformulation technique for the green synthesis of CuNPs using extracts of spices, which showed strong antibacterial activities against S. aureus. These biogenic copper nanoparticles of 150–200 nm in size were further characterized with UV-Vis, scanning electron microscopy (SEM), and EDAX analysis
Summary
There has been a continuing renewal of interest pertaining to the utilization of therapeutic and aromatic plants to produce antimicrobial drugs as plantderived drugs are assured to be harmless and without side-effects [1]. Spices are indispensable components of Indian cuisines They are rich sources of dynamic antimicrobial compounds [2]. In all science fields, including the therapeutic field, metallic nanoparticles are being utilized. They are still alluring scientists to investigate new scopes due to their small sizes. Plant-based molecules are of great interest to the scientific community because they have a wide range of sizes and structures with various biological functions [7]. Copper nanoparticles are broadly used in batteries, optical devices, polymers, and multilayer metal ceramics. They are used for drug delivery and as antimicrobials or antioxidants [8]
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
