Phytoremediation potential of hybrid Pennisetum in cadmium-contaminated soil and its physiological responses to cadmium.

Abstract

Cadmium (Cd) contamination in soil is a global problem. Recently, phytoremediation with plants, possessing high biomass and moderate Cd enrichment ability, has received excessive attention as a cost-effective method for Cd remediation from the soil. In this study, the plant growth, physiological responses, Cd concentration, accumulation, and distribution of the C4 grass hybrid Pennisetum (HP) were studied in different levels of Cd-contaminated soil in a pot experiment. Furthermore, a field trial was also conducted to accurately assess its practical phytoremediation potential in natural Cd-contaminated fallow filed. The results showed that HP possessed effective antioxidant enzymes to scavenge ROS and strong physiological coordination in response to Cd stress. The HP had a considerable Cd enrichment ability, and the maximal Cd uptake of 1.08mg plant-1 was achieved at 60mgkg-1 Cd in the pot. The maximal concentration of Cd in the aboveground parts and roots of HP were 49.33mgkg-1 and 103.33mgkg-1, respectively, when soil Cd was 70mgkg-1 in the pot. The bioconcentration factor (BCF) of Cd in the aboveground parts was less than 1, while the BCF in the root was greater than 1, and the translocation factor (TF) was less than 0.5 in all Cd treatment groups. A total of 46.89-65.46% absorbed Cd stored in the aboveground parts in the pot. The Cd concentration in roots of HP was significantly higher compared to those in leaves and stems, and all BCFs were greater than 1.5 in a lightly Cd-contaminated field (0.35mgkg-1). Furthermore, HP had high aboveground dry biomass up to 54.63 t ha-1 and accumulated 16.13gha-1 Cd in its aboveground parts in the field, which was accounted for about 91.54% of the total Cd extracted by the plant. The soil Cd concentration was reduced by 60.00% after planting HP. Our results suggest that HP is a potential phytoextractor for Cd in lightly Cd-contaminated soil as well as a phytostabilizer under strong Cd stress in the pot.