Phytoconstituents from Curcuma longa L. aqueous ethanol extract and its immunomodulatory effect on diabetic infected rats

Abstract

Background and objectives Curcuma longa L. ( C. longa ) has been shown to possess a wide variety of pharmacological properties against malignant proliferation, inflammation, and parasitosis. Further, C. longa has been reported to possess antioxidant and hypoglycemic activities. The aim of this study was to isolate and elucidate the structure of the phytoconstituents of turmeric and test the hypoglycemic, antioxidant, antibacterial, and anti-inflammatory properties of the alcohol extract of C. longa on diabetic rats infected with Staphylococcus aureus . Materials and methods The extraction and isolation of the constituents of C. longa were performed according to the standard methods of column, paper, and thin-layer chromatography. The chemical structure of the isolated compounds was established by R f , by characterization of acid hydrolysates, by ultraviolet spectral analysis, and by 1 H-NMR spectroscopy. Serum glucose, immunoglobulin E (IgE), nitric oxide, cytokine levels (IL-6, TNF-α, and IL-1β), and blood cell counts (total and differential) were determined. Results and conclusion Three compounds were isolated from the alcohol extract of C. longa . These compounds were identified as apigenin 7- O -rhamnoside 4′- O -glucoside, a new compound isolated for the first time from nature, and 7-methoxyapigenin-6- C- glucoside and N -(3-methoxyphenyl) acetamide, two known compounds isolated from C. longa for the first time. Also, the alcohol extract of C. longa ameliorated the serum glucose, IgE, nitric oxide, and cytokine levels in diabetic infected rats with S. aureus .