Phytochrome-mediated development of mitochondria in the cotyledons of mustard (Sinapis alba L.) seedlings

Abstract

The development of mitochondria from promitochondria is regulated by phytochrome. This conclusion is based on four lines of evidence: 1. The activity of representative mitochondrial marker enzymes (fumarase, EC 4.2.1.2; succinate dehydrogenase, EC 1.3.99.1; cytochrome oxidase, EC 1.9.3.1) is increased by continuous far-red light and (in 2 of the 3 enzymes) by brief red pulses, the effect of which is reversible by brief far-red pulses. These effects do not merely represent a general growth or proliferation of mitochondria already present but specific responses of individual enzymes. Inhibitors of protein synthesis but not of RNA synthesis suppress the increase of these enzyme activities. 2. Continuous far-red light changes some structural properties of the mitochondrial membranes, detectable by an increased requirement of detergent (Triton X-100) for the solubilization of cytochrome oxidase and a more efficient retainment of the matrix enzyme fumarase during isolation of mitochondria. Continuous far-red light increases the apparent buoyant density of mitochondria on a sucrose density gradient. 3. Continuous far-red light has a strong effect on the morphology of the inner mitochondrial membrane system. Electron micrographs from dark-grown cotyledons show arrays of parallel, plate-like cristae while typical plant mitochondria with irregularly oriented sacculi are formed in the light. These responses indicate the involvement of mitochondria in cytophotomorphogenesis during the transition of the cotyledons from dissimilatory to assimilatory metabolism.