Abstract
Summary We have investigated effects of red/far-red light and Ca 2+ on induction of aspartate kinase in Cicer arietinum . Aspartate kinase activity is increased by red light, and this effect is reversed by far-red light. Northern blot analysis showed red light induces AK gene, whereas far-red is ineffective. These results demonstrate that aspartate kinase is positively regulated by phytochrome. Calcium alone mimicked the red light effect and increased the enzyme level as well as gene expression. Red light together with Ca 2+ had a further enhancing effect. Calcium chelating agent, EGTA or calcium channel blocker, verapamil inhibit the red light effect confirming the involvement of calcium in the phytochrome induced aspartate kinase activity.
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