Abstract

Grouper belongs to one of cultivated commodities whose high selling price gives bright prospects. Grouper belongs to one of cultivated commodities whose high selling price gives bright prospects. However, the greatest faced obstacle in this aquaculture industry lies in disease strike brought by bacterium. Synthetic antibiotic use made from chemical compounds gives long-term negative effect and accumulates the chemical compounds into body tissues. Natural material use as an antibacterial is highly recommended to reduce side effect of medicinal effect. The use of antibacterial extract made from A. acuminata tree bark will give new discourse in handling mischievously bacterial strike problem. The study aims to find out chemical compounds made from A. acuminata and to find out solvent and the best dose of A. acuminata inhibition activity as antibacterial against Vibrio harveyi bacterium. Based on research findings, it is known that A. acuminata extract with methanol solvent has the highest inhibition activity compared to ethyl acetate and n-hexane solvent. Then, a concentration of 100% has the highest inhibition activity compared to concentration of 15%, 25%, 50% and 75%. Phytochemistry test result shows that A. acuminata tree bark contains compounds of alkaloid, phenol, flavonoid, steroid, and terpenoids. Based on the findings, it is concluded that A. acuminata contains phenol compound which is able to increase inhibition activity against V. harveyi bacterium.

Highlights

  • IntroductionOne of sea fishery products belonging to leading export commodity is grouper fish.Grouper fish species going through export commodity are rat grouper (Cromileptes altivelis), tiger grouper (Epinephelus fuscoguttatus), mud grouper (Epinephelus coioides), and Napoleon (Heilinus undulates) (Sukardi, 2007)

  • One of sea fishery products belonging to leading export commodity is grouper fish.Grouper fish species going through export commodity are rat grouper (Cromileptes altivelis), tiger grouper (Epinephelus fuscoguttatus), mud grouper (Epinephelus coioides), and Napoleon (Heilinus undulates) (Sukardi, 2007).Market demand on the commodity is stable; even it tends to increase over the years

  • The study aims to find out chemical compounds made from A. acuminata and to find out solvent and the best dose of A. acuminata inhibition activity as antibacterial against Vibrio harveyi bacterium

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Summary

Introduction

One of sea fishery products belonging to leading export commodity is grouper fish.Grouper fish species going through export commodity are rat grouper (Cromileptes altivelis), tiger grouper (Epinephelus fuscoguttatus), mud grouper (Epinephelus coioides), and Napoleon (Heilinus undulates) (Sukardi, 2007). Major obstacle for tiger grouper fish farming is high mortality rate from husbandry to harvest time. One of main causes causing high mortality rate in grouper fish farming is fish disease strike causing mass mortality (Darwisito, 2002). According to Moriaty (1997), disease brought by Vibrio spp bacterium becomes causal factor of mass mortality to breeding grouper. Strike level of V. harveyi bacterium at tiger grouper hatchery may be counted in couple of hours. This Vibrio bacteria strike may damage breeding fish organ and injure skin. One of alternatives to handle this problem is by utilizing bioactive compound from land plant as antibacterial. Research on land plant use from Alstonia acuminate as antibacterial has not been conducted a lot, it needs scientific study on A. acuminata

Active Material Extraction
Polyphenol Compound Analysis
V harveyi Bacterial Culture
Discussion
Antibacterial Compound Activity Test
Findings
Conclusion
Full Text
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